Antibacterial and Antibiofilm Activity of Tea Tree Oil and Coconut Vinegar against Gardnerella Vaginalis
نویسندگان
چکیده
Introduction: Gardnerella vaginalis is a significant pathogen associated more frequently with genital and rarely extragenital infections in women of reproductive age. Few natural antimicrobial agents such as soap nut, garlic, calendula oil are reported to inhibit the growth of G.vaginalis. The present study was intended to investigate the antibacterial and antibiofilm actions of Tea Tree oil and Coconut Vinegar against clinical isolates of G.vaginalis. Material and Methods: Antimicrobial activity of these agents were determined by Disc diffusion method. Minimum inhibitory concentration (MIC) was determined by Agar dilution technique. Biofilm and Antibiofilm activity of G.vaginalis was detected by tissue culture plate method. Results: By the disc diffusion method, 73.6% were inhibited by Tea tree oil and 80.2% by Vinegar. The MIC range of Tea tree oil and Vinegar varied from 0.78 to 12.5ul/ml. The minimum biofilm inhibition concentration (BIC50) was determined at 200ul/ml concentration. Conclusion: Tea tree oil and Vinegar exhibited good antibacterial and antibiofilm activitiy against G.vaginalis.
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